Consumption of propionate, a food ingredient that’s widely used in baked goods, animal feeds, and artificial flavorings, appears to increase levels of several hormones that are associated with risk of obesity and diabetes.
Propionate is a potent inhibitor of molds, is a short-chain fatty acid that is widely used as a food preservative and is also endogenously produced by gut microbiota.
Propionate is the conjugate base of propionic acid; a key precursor in lipid biosynthesis.
It has a role as a human metabolite.
A new research led by Harvard T.H. Chan School of Public Health in collaboration with researchers from Brigham and Women’s Hospital and Sheba Medical Center in Israel which combined data from a randomized placebo-controlled trial in humans and mouse studies, indicated that propionate can trigger a cascade of metabolic events that leads to insulin resistance and hyperinsulinemia—a condition marked by excessive levels of insulin.
The findings also showed that in mice, chronic exposure to propionate resulted in weight gain and insulin resistance.
“Understanding how ingredients in food affect the body’s metabolism at the molecular and cellular level could help us develop simple but effective measures to tackle the dual epidemics of obesity and diabetes,” said Gökhan S. Hotamisligil, James Stevens Simmons Professor of Genetics and Metabolism and Director of the Sabri Ülker Center for Metabolic Research at Harvard Chan School.
More than 400 million people worldwide suffer from diabetes, and the rate of diabetes incidence is projected to increase 40% by 2040 despite extensive efforts to curb the disease.
The surging rates of diabetes, as well as obesity, in the last 50 years indicate that environmental and dietary factors must be influencing the growth of this epidemic.
Researchers have suggested that dietary components including ingredients used for preparation or preservation of food may be a contributing factor, but there is little research evaluating these molecules.
For this study, the researchers focused on propionate, a naturally occurring short-chain fatty acid that helps prevents mold from forming on foods.
They first administered this short chain fatty acid to mice and found that it rapidly activated the sympathetic nervous system, which led to a surge in hormones, including glucagon, norepinephrine, and a newly discovered gluconeogenic hormone called fatty acid-binding protein 4 (FABP4).
This in turn led the mice to produce more glucose from their liver cells, leading to hyperglycemia—a defining trait of diabetes.
Moreover, the researchers found that chronic treatment of mice with a dose of propionate that was equivalent to the amount typically consumed by humans led to significant weight gain in the mice, as well as insulin resistance.
To determine how the findings in mice may translate to humans, the researchers established a double-blinded placebo-controlled study that included 14 healthy participants.
The participants were randomized into two groups: one group received a meal that contained one gram of propionate as an additive and the other group was given a meal that contained a placebo.
Blood samples were collected before the meal, within 15 minutes of eating the meal, and every 30 minutes thereafter for four hours.
The researchers found that people who consumed the meal containing propionate had significant increases in norepinephrine as well as increases in glucagon and FABP4 soon after eating the meal.
The findings indicate that propionate may act as a “metabolic disruptor” that potentially increases the risk for diabetes and obesity in humans.
The researchers noted that while propionate is generally recognized as safe by the U.S. Food and Drug Administration, these new findings warrant further investigation into propionate and potential alternatives that could be used in food preparation.
“The dramatic increase in the incidence of obesity and diabetes over the past 50 years suggests the involvement of contributing environmental and dietary factors.
One such factor that warrants attention is the ingredients in common foods.
We are exposed to hundreds of these chemicals on a daily basis, and most have not been tested in detail for their potential long-term metabolic effects,” said Amir Tirosh, MD, PhD, of the Brigham’s Division of Endocrinology, Diabetes and Hypertension.
Tirosh is also an associate professor of medicine at Tel-Aviv University’s Sackler School of Medicine, director of the Division of Endocrinology at Sheba Medical Center in Israel, and research fellow at Harvard Chan School.
The study was published online in Science Translational Medicine on April 24, 2019.
Other authors included Ediz S. Calay, Gurol Tuncman, Kathryn C. Claiborn, Karen E. Inouye, Kosei Eguchi, Michael Alcala, Moran Rathaus, Kenneth S. Hollander, Idit Ron, Rinat Livne, Yoriko Heianza, Lu Qi, Iris Shai, Rajesh Garg, and Gökhan S. Hotamisligil.
For more information
Science Translational Medicine
The short-chain fatty acid propionate increases glucagon and FABP4 production, impairing insulin action in mice and humans
Brigham and Women’s Hospital
Sheba Medical Center
Harvard T.H. Chan School of Public Health
This post is also available in: Italian